Damping of liquid sloshing by foams: from everyday observations to liquid transport

We perform experiments on the sloshing dynamics of liquids in a rectangular container submitted to an impulse. We show that when foam is placed on top of the liquid the oscillations of the free interface are significantly damped. The ability to reduce sloshing and associated splashing could find applications in numerous industrial processes involving liquid transport.Comment: Accepted for publication in Journal of Visualizatio

Microfluidic In Situ Measurement of Poisson's Ratio of Hydrogels

Being able to precisely characterize the mechanical properties of soft microparticles is essential for numerous situations from the understanding of the flow of biological fluids to the development of soft micro-robots. Here we present a simple measurement technique for the Poisson's ratio of soft micron-sized hydrogels in the presence of a surrounding liquid. This methods relies on the measurement of the deformation in two orthogonal directions of a rectangular hydrogel slab compressed uni-axially inside a microfluidic channel. Due to the in situ character of the method, the sample does not need to be dried, allowing for the measurement of the mechanical properties of swollen hydrogels. Using this method we determine the Poisson's ratio of hydrogel particles composed of polyethylene glycol (PEG) and varying solvents fabricated using a lithography technique. The results demonstrate with high precision the dependence of the hydrogel compressibility on the solvent fraction and character. The method, easy to implement, can be adapted for the measurement of a variety of soft and biological materials

The recombinase protein is a torque sensitive molecular switch

How a nano-searcher finds its nano-target is a general problem in non-equilibrium statistical physics. It becomes vital when the searcher is a damaged DNA fragment trying to find its counterpart on the intact homologous chromosome. If the two copies are paired, that intact homologous sequence serves as a template to reconstitute the damaged DNA sequence, enabling the cell to survive without genetic mutations. To succeed, the search must stop only when the perfect homology is found. The biological process that ensures such a genomic integrity is called Homologous Recombination and is promoted by the Recombinase proteins. In this article, we use torque-sensitive magnetic tweezers to measure the free-energy landscape of the human Recombinase hRad51 protein assembled a DNA fragment. Based on our measurements we model the hRad51/DNA complex as an out-of-equilibrium two-state system and provide a thermodynamical description of Homologous Recombination. With this dynamical two-state model, we suggest a mechanism by which the recombinase proteins discriminate between homologous and a non-homologous sequences

Stress Clamp Experiments on Multicellular Tumor Spheroids

The precise role of the microenvironment on tumor growth is poorly understood. Whereas the tumor is in constant competition with the surrounding tissue, little is known about the mechanics of this interaction. Using a novel experimental procedure, we study quantitatively the effect of an applied mechanical stress on the long-term growth of a spheroid cell aggregate. We observe that a stress of 10 kPa is sufficient to drastically reduce growth by inhibition of cell proliferation mainly in the core of the spheroid. We compare the results to a simple numerical model developed to describe the role of mechanics in cancer progression.Comment: 5 pages, 4 figure

Beads, bubbles and drops in microchannels: stability of centered position and equilibrium velocity

Understand and predict the dynamics of dispersed micro-objects in microfluidics is crucial in numerous natural, industrial and technological situations. In this paper, we experimentally characterized the equilibrium velocity $V$ and lateral position $\varepsilon$ of various dispersed micro-objects such as beads, bubbles and drops, in a cylindrical microchannel over an unprecedent wide range of parameters. By systematically varying the dimensionless object size ($d \in [0.1; 1]$), the viscosity ratio ($\lambda \in [10^{-2}; \infty[$), the density ratio ($\varphi \in [10^{-3}; 2]$), the Reynolds number ($\Re \in [10^{-2}; 10^2]$), and the capillary number ($\text{Ca} \in [10^{-3}; 0.3]$), we offer a general study exploring various dynamics from the nonderformable viscous regime to the deformable visco-inertio-capillary regime, thus enabling to highlight the sole and combined roles of inertia and capillary effects on lateral migration. The experiments are compared and well-agree with a steady 3D Navier-Stokes model for incompressible two-phase fluids including both the effects of inertia and possible interfacial deformations. This model enables to rationalize the experiments and to provide an exhaustive parametric analysis on the influence of the main parameters of the problem, mainly on two aspects: the stability of the centered position and the velocity of the dispersed object. Interestingly, we propose a useful correlation for the object velocity $V$ as functions of the $d$, $\varepsilon$ and $\lambda$, obtained in the $\text{Re}=\text{Ca}=0$ limit, but actually valid for a larger range of values of $\text{Re}$ and $\text{Ca}$ in the linear regimes.Comment: 22 pages, 11 figures, submitted to Journal of Fluid Mechanic

Probing Rad51-DNA interactions by changing DNA twist

In eukaryotes, Rad51 protein is responsible for the recombinational repair of double-strand DNA breaks. Rad51 monomers cooperatively assemble on exonuclease-processed broken ends forming helical nucleo-protein filaments that can pair with homologous regions of sister chromatids. Homologous pairing allows the broken ends to be reunited in a complex but error-free repair process. Rad51 protein has ATPase activity but its role is poorly understood, as homologous pairing is independent of adenosine triphosphate (ATP) hydrolysis. Here we use magnetic tweezers and electron microscopy to investigate how changes of DNA twist affect the structure of Rad51-DNA complexes and how ATP hydrolysis participates in this process. We show that Rad51 protein can bind to double-stranded DNA in two different modes depending on the enforced DNA twist. The stretching mode is observed when DNA is unwound towards a helical repeat of 18.6 bp/turn, whereas a non-stretching mode is observed when DNA molecules are not permitted to change their native helical repeat. We also show that the two forms of complexes are interconvertible and that by enforcing changes of DNA twist one can induce transitions between the two forms. Our observations permit a better understanding of the role of ATP hydrolysis in Rad51-mediated homologous pairing and strand exchang

Mechanical behavior of multi-cellular spheroids under osmotic compression

The internal and external mechanical environment plays an important role in tumorogenesis. As a proxy of an avascular early state tumor, we use multicellular spheroids, a composite material made of cells, extracellular matrix and permeating fluid. We characterize its effective rheology at the timescale of minutes to hours by compressing the aggregates with osmotic shocks and modeling the experimental results with an active poroelastic material that reproduces the stress and strain distributions in the aggregate. The model also predicts how the emergent bulk modulus of the aggregate as well as the hydraulic diffusion of the percolating interstitial fluid are modified by the preexisting active stress within the aggregate. We further show that the value of these two phenomenological parameters can be rationalized by considering that, in our experimental context, the cells are effectively impermeable and incompressible inclusions nested in a compressible and permeable matrix

Dichloroacetate prevents cisplatin-induced nephrotoxicity without compromising cisplatin anticancer properties

Cisplatin is an effective anticancer drug; however, cisplatin use often leads to nephrotoxicity, which limits its clinical effectiveness. In this study, we determined the effect of dichloroacetate, a novel anticancer agent, in a mouse model of cisplatin-induced AKI. Pretreatment with dichloroacetate significantly attenuated the cisplatin-induced increase in BUN and serum creatinine levels, renal tubular apoptosis, and oxidative stress. Additionally, pretreatment with dichloroacetate accelerated tubular regeneration after cisplatin-induced renal damage. Whole transcriptome sequencing revealed that dichloroacetate prevented mitochondrial dysfunction and preserved the energy-generating capacity of the kidneys by preventing the cisplatin-induced downregulation of fatty acid and glucose oxidation, and of genes involved in the Krebs cycle and oxidative phosphorylation. Notably, dichloroacetate did not interfere with the anticancer activity of cisplatin in vivo. These data provide strong evidence that dichloroacetate preserves renal function when used in conjunction with cisplatin